Congratulations! J. Ed. Anschließend fügt die Thymidylatsynthase eine Methylgruppe (-CH 3), die von N 5,N 10-Methylen-THF geliefert wird, an den Pyrimidinring des dUMP. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives.
The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. C1141, C1145, U1431, U1432.
A password reset email has been sent to the primary email address associated with your account. Nucleotide Selector for DNA/RNA Labeling (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Terms and Conditions Chem. Please update your browser to Internet Explorer 11 or above. Angew.  Burley et al. Trademarks. Adding dUTP results in PCR products with U instead of T. If this product is carried over into a new PCR, it might outcompete the intended DNA template. Let's find the product that meets your needs. Soc. All Rights Reserved.
Available as kit, master mix or standalone enzyme. © 2020 Promega Corporation. To protect your privacy, your account has been locked after 6 failed login attempts. dUTP is used to replace TTP in the PCR reaction so that all PCR products will contain dUTP.
2000 Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dUTP (2´-Deoxyuridine, 5´-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. When you select your country, you agree that we can place these functional cookies on your device. You have not verified your email address. There was an issue sending the verification email. Eur. Please try again or contact Customer Service.
There was an issue verifying your email address. Ready-to-use optimized master mix for room-temperature PCR assembly.
dNTP Mix. A nucleoside triphosphate is a molecule containing a nitrogenous base bound to a 5-carbon sugar (either ribose or deoxyribose), with three phosphate groups bound to the sugar. Get in touch with a nearby distributor or sales representative, Catalog number selected: Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL) Our website uses functional cookies that do not collect any personal information or track your browsing activity. Your password reset link has expired. Application Digoxigenin-11-dUTP, alkali-stable has been used to label probes for fluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR) Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached.
128 (5):1398. High-performance hot-start GoTaq® G2 enzyme with convenient room-temperature setup. A verified email address is required to access the full functionality of your Promega.com account. Daher ist das Mischungsverhältnis zwischen dTTP und dUTP für den Erfolg der PCR entscheidend - und wird dementsprechend von den meisten Firmen, die eine Nucleotidmischung anbieten, nicht genau angegeben. Our customer and technical support experts are here to help! 48:9879. U1511, U1515. Current Protocols in Chemical Biology 3:153. It is an example of a nucleotide.They are the molecular precursors of both DNA and RNA, which are chains of nucleotides made through the processes of DNA replication and transcription. Der Ausfall von Enzymen, die am Umsatz der Nucleotide beteiligt sind, ... Da nur das Monophosphat methyliert wird, wird dUDP zunächst zu dUTP phosphoryliert und nun ein Pyrophosphat abgespalten, sodass dUMP entsteht. For research use only! Please try again or contact Customer Service.
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